HPLC Column, RP C4 Butyl, Wide Pore, 10um, 4.6mm ID x 100mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. Classified as USP L26, this stationary phase provides moderate hydrophobic retention while offering enhanced accessibility for larger molecules through its 300 Å pore structure. The C4 chemistry is widely used for proteins, peptides, enzymes, and other biomolecules that require efficient mass transfer and reduced hydrophobic retention compared to longer alkyl-chain phases.
68004-10P-3M is a 100 mm x 4.6 mm ID analytical HPLC column packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The 100 mm length provides an excellent balance between chromatographic resolution and analysis time, making it well suited for routine bioanalytical methods, protein characterization, and peptide separations. The 10 µm particle size supports higher flow rates with lower operating backpressure, while the 4.6 mm ID format is the industry standard for conventional HPLC systems and typically operates at approximately 1.0–2.0 mL/min.
This USP L26 column is particularly useful for the separation and characterization of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. The combination of fully endcapped C4 chemistry, high-purity silica, wide pore architecture, and larger particle size provides excellent recovery, efficient mass transfer, and reliable chromatographic performance for demanding bioanalytical and biomolecule analysis applications.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
USP L26 classification
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
300 Å wide pore structure for biomolecules
High-purity silica support
10 µm particles for lower backpressure
Suitable for higher flow rates
Excellent for protein and peptide separations
Balanced speed and resolution with 100 mm length
Typical Applications
Reversed-phase HPLC
USP L26 methods
Protein analysis
Peptide analysis
Enzyme characterization
Bioanalytical methods
Biomolecule separations
Method development
Quality control testing
Large molecule analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 4.6 mm ID x 100 mm
Length: 100 mm
Recommended Flow Rate: 1.0–2.0 mL/min
Brand: Cogent
Important Notes
Designed for reversed-phase chromatography of proteins and other large molecules
Wide pore structure enhances analyte access to the stationary phase
Provides less hydrophobic retention than comparable C8 columns
Larger particle size reduces operating backpressure
Excellent choice for bioanalytical and biomolecule characterization applications
Compatible with conventional HPLC systems
Alternative for many USP L26 methods